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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
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Published on: August 25, 2018

[Fluorometric method for determination of keratin SH-groups in human epidermis]

S A Morenkova, L G Nagler

    Biomeditsinskaia Khimiia
    |June 11, 2005
    PubMed

    Abstract:

    Simple and highly sensitive fluorometric method for determination of keratin SH-groups using fluorescein mercuric acetate has been developed. It allows to determine amount of SH-groups up to 0.1 nmole/mg of protein in extracts of corneal layer of human epidermis.

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    Cells of the Epidermis01:24

    Cells of the Epidermis

    The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
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