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Updated: Aug 17, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Review: derivatization in mass spectrometry--5. Specific derivatization of monofunctional compounds
John M Halket1, Vladimir G Zaikin
1Drug Control Centre, King's College London, Franklin-Wilkins Building, Stamford Street, London SE1 9NH, UK. john.halket@kcl.ac.uk
Abstract:
The present paper is complementary to the foregoing reviews and describes some additional methods of the derivatization of particular functional groups mainly to enhance the structural information content of electron ionization and chemical ionization mass spectra. Derivatization approaches for the modification of unsaturated compounds, alcoholic, carboxylic, carbonyl, amine and other functional groups, are discussed. Derivatization for separation and quantitative determination of chiral enantiomeric compounds is also considered. Preliminary chemical and physicalchemical degradation for structure elucidation of high molecular weight compounds (biopolymers, synthetic polymers) is mentioned. Chemical aspects of derivatizations and characteristic mass spectral features of derivatives are described briefly. Some particular applications of chemical modification, in conjunction with mass spectral measurements for the analysis of various important bioorganic compounds and compounds in biological fluids, air, environmental etc., are considered.
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