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Related Experiment Videos

Ex vivo analysis of T-cell function.

Maria A Suni1, Vernon C Maino, Holden T Maecker

  • 1Becton, Dickinson and Company Biosciences, 2350 Qume Drive, San Jose, California 95131, USA.

Current Opinion in Immunology
|June 14, 2005
PubMed
Summary

Recent advancements allow detailed analysis of T-cell functions, from early signaling to proliferation. New methods like CD107 detection and polychromatic flow cytometry enhance T-cell response assessment.

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Area of Science:

  • Immunology
  • Cellular Biology
  • Biotechnology

Background:

  • T-cell analysis in vitro has evolved significantly.
  • Assays now cover early signaling, intermediate functions, and proliferation.

Purpose of the Study:

  • To review recent advancements in T-cell function analysis.
  • To highlight novel methods for assessing T-cell responses.

Main Methods:

  • Analysis of T-cell signaling events (e.g., phosphorylation).
  • Measurement of intermediate functions (degranulation, cytokine production).
  • Assessment of later functions (proliferation).
  • Utilizing cell surface CD107 exposure for degranulation analysis.
  • Employing polychromatic flow cytometry for multi-parameter T-cell analysis.

Main Results:

  • Significant progress in analyzing T-cell functions in vitro.
  • CD107 exposure is a key indicator of T-cell degranulation.
  • Polychromatic flow cytometry enables simultaneous assessment of multiple T-cell phenotypes and functions.

Conclusions:

  • Current in vitro assays provide comprehensive analysis of T-cell functions.
  • Novel techniques like CD107 detection and polychromatic flow cytometry improve the depth and breadth of T-cell response evaluation.

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