Determination of hydrogen peroxide scavenging activity of cinnamic and benzoic acids employing a highly sensitive peroxyoxalate chemiluminescence-based assay: structure-activity relationships
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Updated: Aug 17, 2026

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
Published on: July 28, 2016
Abdelhak Mansouri1, Dimitris P Makris, Panagiotis Kefalas
1Department of Food Quality Management & Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania (MAICh), Greece.
Abstract:
A series of cinnamic acids along with their corresponding benzoate analogues were tested for their ability to scavenge hydrogen peroxide (H(2)O(2)), by using a highly sensitive, peroxyoxalate chemiluminescence assay. Among benzoic acid derivatives, vanillic acid (3-hydroxy-4-methoxybenzoic acid) was found to be the most efficient H(2)O(2) scavenger with its hydrogen peroxide scavenging activity (SA(HP)) being 170.20 microM(-1), whereas protocatechuic acid (3,4-dihydroxybenzoic acid) exhibited the weakest activity (5.90 microM(-1)). Caffeic acid (3,4-dihydroxycinnamic acid) was the strongest antioxidant amongst cinnamate derivatives with a SA(HP) = 8.2 microM(-1), as opposed to m-coumaric acid (2-hydroxycinnamic acid), which was found to be a poor hydrogen peroxide scavenger (SA(HP) = 0.18 microM(-1)). Comparison between the two groups revealed that benzoate derivatives are much stronger hydrogen peroxide quenchers in relation with their cinnamate analogues, and this finding was discussed on a basis of structure-activity relationships and comparative assessment of other antioxidant characteristics.
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