Related Experiment Video
Updated: Aug 6, 2026

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
Published on: October 24, 2011
Three-factor response surface optimization of the production of dextran dextrinase by Gluconobacter oxydans
M Naessens1, R Vercauteren, E J Vandamme
1Department of biochemical and microbial technology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
Abstract:
Response surface methodology (RSM) and a five-level three-factor central composite rotatable design (CCRD) were used to evaluate the effect of glycerol and peptone concentration and initial pH on dextran dextrinase (DDase) production by Gluconobacter oxydans. Optimal fermentation conditions were 20.59 g/l of glycerol, 6.67 g/l of mycological peptone and an initial pH of 6.14. The predicted DDase yield of the optimised fermentation was 0.207 U/ml, whereas an actual experimental yield of 0.208 +/- 0.025 U/ml was obtained.
Related Concept Videos
Bioreactor Controls-III
Methods of Medium Optimization
Upstream Processing
Production of Organic Acids
Production of Pharmaceuticals

