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Updated: Aug 17, 2026

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
Published on: June 11, 2010
[Transient expression of GUS gene controlled by different regulator sequences in rice and Populus tomrutosa]
Gen-Qi Liu1, Xiu-Li Li, Chun-Ling Yang
1Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing 100101, China. genqiliu@sina.com.cn
Abstract:
GUS gene controlled by several kinds of regulator sequences (promoters, TMVOmega enhancer and fragments of 18S rRNA gene from Arabidopsis thaliane) were introduced into the calli of rice and Populus tomrutosa respectively by gene gun. The transient expression of GUS gene in the calli of rice and P. tomrutosa was investigated. The results showed: (1) in the calli of rice, Ubil promoter was the most effective one among the several regulator sequences and CaMV35S with fragments of 18S rRNA gene is the least effective one; (2) in the calli of P. tomrutosa, CaMV35S promoter with 18S rDNA fragments was the most effective one among the several regulator sequences; and (3) tandem CaMV35S-Ubil promoter with TMVOmega sequence could not enhance significantly the expression of GUS gene in the calli of rice and P. tomrutosa.
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