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Related Experiment Videos

Esf2p, a U3-associated factor required for small-subunit processome assembly and compaction.

Tran Hoang1, Wen-Tao Peng, Emmanuel Vanrobays

  • 1Fonds National de la Recherche Scientifique, Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires, Charleroi-Gosselies, Belgium.

Molecular and Cellular Biology
|June 21, 2005
PubMed
Summary
This summary is machine-generated.

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Esf2p protein depletion inhibits early pre-ribosome processing and 18S rRNA synthesis in yeast. This impairs small subunit processome assembly and U3 snoRNA release, suggesting a conserved role for ABT1 in mammals.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Esf2p, the yeast homolog of mouse ABT1, was previously thought to interact with TATA-binding protein.
  • Large-scale studies indicate Esf2p localizes to the nucleolus and associates with pre-ribosome processing factors.

Purpose of the Study:

  • To investigate the role of Esf2p in pre-ribosome processing and 18S rRNA synthesis in Saccharomyces cerevisiae.
  • To determine the molecular mechanism by which Esf2p functions in the nucleolus.

Main Methods:

  • Esf2p depletion in yeast cells.
  • Analysis of pre-rRNA processing intermediates using Northern blotting and glycerol gradient centrifugation.
  • Chromatin spreading to assess small subunit (SSU) processome assembly.

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Main Results:

  • Esf2p-depleted cells exhibit defects in early pre-rRNA processing (cleavage sites A0-A2) and reduced 18S rRNA synthesis.
  • Esf2p associates with 5' external transcribed spacer (ETS) and U3 small nucleolar RNA (snoRNA) within the SSU processome/90S preribosomes.
  • Esf2p depletion inhibits SSU processome assembly and compaction, trapping U3 snoRNA within 90S preribosomes.

Conclusions:

  • Esf2p is essential for early pre-ribosome processing and efficient 18S rRNA synthesis.
  • Proper assembly of the SSU processome and release of U3 snoRNA depend on Esf2p function.
  • The role of Esf2p/ABT1 in ribosome biogenesis may be conserved across species, including mammals.