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Updated: Aug 17, 2026
Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions
Published on: July 22, 2013
A reagentless biosensor of nitric oxide based on direct electron transfer process of cytochrome c on multi-walled
Guang-Chao Zhao1, Zheng-Zhi Yin, Xian-Wen Wei
1Anhui Key Laboratory of Functional molecular Solids, School of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China. gczhao@mail.ahnu.edu.cn
Abstract:
Direct electron transfer between Cytochrome c (Cyt.c) and electrode can be achieved through immobilizing Cyt.c on the surface of multi-walled carbon nanotubes (MWNTs). Under the condition of cyclic potential scans, Cyt.c can be adsorbed on the surface of MWNTs that were modified on a glassy carbon (GC) electrode to form an approximate monolayer. The redox characteristic and bioactivity of Cyt.c could be remained after it was adsorbed on MWNTs' surface. This provides a way to construct a new biosenser based on the activity of Cyt.c. Further investigation displayed that Cyt.c adsorbed on MWNTs showed an enzyme-like activity to catalyze the reduction of nitric oxide (NO). Due to catalyzing by Cyt.c, the reduction of NO in aqueous solution was achieved, which reductive potential appeared at -0.747V (vs. SCE). The peak currents were linearly proportional to concentration of NO in the range from 2 to 48 micromol/l with a limit of detection of 1.3 microM. The biosensor showed a good stability and excellent repeatability.
