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Mouse amelogenin exons 8 and 9: sequence analysis and protein distribution
P Papagerakis1, J M Ibarra, N Inozentseva
1Department of Pediatric Dentistry, Dental School, University of Texas Health Science Center San Antonio, MSC 7888, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.
Journal of Dental Research
|June 24, 2005
Summary
Researchers identified novel mouse amelogenin exons 8/9 sequences, revealing alternative splicing and localization in ameloblasts and odontoblasts during tooth development.
Area of Science:
- Biochemistry
- Developmental Biology
- Genetics
Background:
- Amelogenin is the primary protein in developing enamel.
- Rat amelogenin has two additional characterized exons, 8 and 9.
Purpose of the Study:
- Identify mouse amelogenin exons 8/9 sequences.
- Investigate alternative spliced isoforms of amelogenin exons 8/9.
- Immunolocalize proteins encoded by exons 8/9 during odontogenesis.
Main Methods:
- RT-PCR analysis using mouse tooth cDNA and dental cell lines.
- DNA sequence analysis.
- Immunohistochemistry.
Main Results:
- Identified mouse amelogenin exons 8/9 sequences with 93% identity to rat sequences.
- Detected alternative splicing of exon 3 in cDNAs lacking exons 8/9.
- Localized proteins containing exon 8/9 sequences in ameloblasts, young odontoblasts, and stratum intermedium cells.
Conclusions:
- The mouse AMELX gene contains two additional exons (8/9).
- Amelogenin 8/9 is synthesized by ameloblasts and odontoblasts.
- Amelogenin splice variants may possess distinct roles in tooth formation.