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Updated: Aug 17, 2026
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
Ultrasonically nebulized distilled water prevents exogenous histamine hyperreactivity in Toxocara canis-infected mice
A Sá-Nunes1, R M F Bolzoni, A I Medeiros
1Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, 14040-903, São Paulo, Brazil.
Objective:
This study examines the effect of ultrasonically nebulized distilled water inhalation on the systemic histamine hyperreactivity of Toxocara canis-infected mice.
Methods:
Uninfected and T. canis-infected mice received an intravenous sublethal dose of histamine and lethality rates were documented. At 24 days post infection, infected mice received ultrasonically nebulized distilled water inhalation for 1 h. Twenty-four hours later histamine levels were determined in bronchoalveolar lavage fluid as well as histamine lethality and toluidine blue-stained mast cell number in the lung.
Results:
T. canis-infected mice showed increased lethality after exposure to histamine in comparison to uninfected mice. Ultrasonically nebulized distilled water inhalation prevented histamine-induced lethality and reduced toluidine blue-stained mast cell numbers in the lung.
Conclusions:
The correlation between decreases in stained mast cells in the lung after ultrasonically nebulized distilled water inhalation and inhibition of histamine-induced lethality in these animals suggests participation of mast cells in the phenomenon and could be helpful in understanding the mechanisms of hyperreactivity during helminth parasite infections.
