Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Betaine improves LA-PCR amplification.

Xu-Qing Chen1, Xiao-Dong Zhang, Rong-Qi Liang

  • 1Beijing Agro-Biotechnology Research Center, Beijing 100089, China. chenxvqing@sina.com

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|June 25, 2005
PubMed
Summary

Betaine significantly enhances long DNA amplification using Polymerase Chain Reaction (PCR). This optimization is crucial for amplifying intact genes needed for effective gene expression and constructing specialized vectors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Piezo channel is central to the mechano-sensitive channel complex in the mammalian inner ear.

Research square·2023
Same author

Coherent control of a high-orbital hole in a semiconductor quantum dot.

Nature nanotechnology·2023
Same author

Ilaprazole-amoxicillin dual therapy at high dose as a first-line treatment for helicobacter pylori infection in Hainan: a single-center, open-label, noninferiority, randomized controlled trial.

BMC gastroenterology·2023
Same author

Heat shock protein family A member 8 serving as a co-activator of transcriptional factor ETV4 up-regulates PHLDA2 to promote the growth of liver cancer.

Acta pharmacologica Sinica·2023
Same author

Emerging nanotechnology for Alzheimer's disease: From detection to treatment.

Journal of controlled release : official journal of the Controlled Release Society·2023
Same author

Tracking tumor heterogeneity and progression with near-infrared II fluorophores.

Exploration (Beijing, China)·2023

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Polymerase Chain Reaction (PCR) is vital for DNA amplification but struggles with long DNA segments.
  • Intact genes are essential for specific and effective gene expression.
  • Amplifying long DNA sequences is necessary for constructing specialized expression vectors.

Purpose of the Study:

  • To optimize the Polymerase Chain Reaction (PCR) system for amplifying long DNA targets.
  • To identify chemical additives that improve the efficiency and specificity of long-range PCR.
  • To evaluate the effectiveness of betaine in enhancing the amplification of intact genes.

Main Methods:

  • Systematic testing of various chemical additives to optimize the long-range PCR system.
  • Investigating the effect of betaine concentration on DNA amplification.

Related Experiment Videos

  • Assessing the amplification of long DNA segments (9 kb and 16 kb) from plant and plasmid DNA.

    • Main Results:

    • Betaine was identified as the most effective additive for optimizing long-range PCR.
    • Optimal betaine concentrations ranged from 1.0 mol/L to 2.5 mol/L.
    • Betaine successfully amplified a 9 kb maize DNA segment and a 16 kb plasmid DNA segment, reducing non-specific amplification.

    Conclusions:

    • Betaine significantly improves the amplification of long DNA targets, including intact genes.
    • The optimal betaine concentration is dependent on primer and target DNA characteristics (e.g., GC content).
    • Betaine is a valuable tool for enhancing long-range PCR, facilitating gene cloning and expression vector construction.