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Published on: May 23, 2025
[Expression, purification and enzymatic characterization of Bacillus polymyxa beta-glucosidase gene( bglA ) in
Yun Zhao1, Wei-Feng Liu, Ai-Jun Mao
1Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Abstract:
The beta-glucosidase encoding gene bglA was cloned from Bacillus polymyxa 1.794. The bglA gene was inserted in expression vector pET28a(+) and transformed into Escherichia coli BL21 (DE3), finally the recombinant strain BL1979 was obtained. Induced by IPTG, the expression P-glucosidase activity reached to 24.7 IU/mL. The optimum temperature and optimum pH of the recombinant expression P-glucosidase in BL1979 were 37 degrees C and 7.0 respectively,the purity can reach to 92.7%. Analysis of the fusion protein by nondenaturing gradient gel electrophoresis, we found the fusion protein exists in dimmer, tetramer,hexamer and octamer, they all have hydrolase activity.
