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Calibration of a detector for nonlinear chromatography
Leonid Asnin1, Wilmer Galinada, Gustaf Götmar
1Department of Chemistry, The University of Tennessee, 552 Buehler Hall, Knoxville, TN 37996-1600, USA.
Journal of Chromatography. A
|June 25, 2005
Summary
This study presents a new method for calibrating chromatography detectors using peak responses instead of traditional plateau methods. This approach conserves valuable sample material, crucial for nonlinear chromatography applications.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Nonlinear chromatography often requires detector calibration due to high solute concentrations.
- Standard calibration methods use large sample volumes, which can be impractical or costly.
Purpose of the Study:
- To develop and validate an alternative calibration method for chromatography detectors.
- To reduce the sample volume required for detector calibration in preparative chromatography.
Main Methods:
- Utilized a series of injection pulses with increasing concentrations.
- Recorded peak responses from the detector for each pulse.
- Developed a calibration curve based on these peak responses.
Main Results:
- Successfully generated a calibration curve using peak heights.
- Demonstrated the validity of the peak-based calibration method.
- Reduced the need for large volumes of pure solutes for calibration.
Conclusions:
- The proposed method offers an efficient alternative for detector calibration in chromatography.
- This technique is particularly beneficial when dealing with limited or expensive sample materials.
- Validates a new approach for accurate concentration determination in preparative chromatography.