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Preparative isolation and characterization of some minor impurities of astaxanthin by high-performance liquid
R Nageswara Rao1, S Naseeruddin Alvi, B Nageswara Rao
1Indian Institute of Chemical Technology, Hyderabad 500007, India. rnrao@ins.iictnet.com
Abstract:
A simple and rapid preparative high-performance liquid chromatography (HPLC) method has been developed to isolate and characterize some minor impurities of astaxanthin using a normal-phase Lichrosorb silica column with n-hexane-acetone-tetrahydrofuran (90:2:8, v/v/v) as mobile-phase and detection at 475 nm. The isolated impurities were characterized as astacene, dehydro astacene and apoastaxanthinal by UV-vis, ESI-MS, 1H and 13C NMR spectroscopy and the molecular structures were assigned. The impurities collected using the developed conditions were over 98% pure.
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