Errors of measurement for blood volume parameters: a meta-analysis

The volume of red blood cells (V(RBC)) is used routinely in the diagnostic workup of polycythemia, in assessing the efficacy of erythropoietin administration, and to study factors affecting oxygen transport. However, errors of various methods of measurement of V(RBC) and related parameters are not well characterized. We meta-analyzed 346 estimates of error of measurement of V(RBC) for techniques based on Evans blue (V(RBC,Evans)), 51chromium-labeled red blood cells (V(RBC,51Cr)), and carbon monoxide (CO) rebreathing (V(RBC,CO)), as well as hemoglobin mass with the carbon-monoxide method (M(Hb,CO)), in athletes and active and inactive subjects undergoing various experimental and control treatments lasting minutes to months. Subject characteristics and experimental treatments had little effect on error of measurement, but measures with the smallest error showed some increase in error with increasing time between trials. Adjusted to 1 day between trials and expressed as coefficients of variation, mean errors for M(Hb,CO) (2.2%; 90% confidence interval 1.4-3.5%) and V(RBC,51Cr) (2.8%; 2.4-3.2%) were much less than those for V(RBC,Evans) (6.7%; 4.9-9.4%) and V(RBC,CO) (6.7%; 3.4-14%). Most of the error of V(RBC,Evans) was due to error in measurement of volume of plasma via Evans blue dye (6.0%; 4.5-7.8%), which is the basis of V(RBC,Evans). Most of the error in V(RBC,CO) was due to estimates from laboratories with a relatively large error in M(Hb,CO), the basis of V(RBC,CO). V(RBC,51Cr) and M(Hb,CO) are the best measures for research on blood-related changes in oxygen transport. With care, V(RBC,Evans) is suitable for clinical applications of blood-volume measurement.