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[Study on ultrasonic extraction technics of oleanolic acid in Akebia trifoliata]
Weiguo Liu1, Wenyu Yang, Qiyin Xiao
1Sichuan Agriculture University, Ya'an 625014.
Objective:
To optimize the ultrasonic extraction condition for oleanolic acid from Akebia trifoliata.
Method:
According to the orthogonal design L9 (3(4)), the optimum ultrasonic extraction technique was sought through experimental investigation, and was compared with the routine extraction technics. The contents of oleanclic acid were determined by UV-spectrophotometry.
Result:
The order of factors to affect the oleanolic acid extraction was extraction time > ethanol concentration > ultrasonic power > ration of interval time to ultrasonic time. The optimum ultrasonic extractions were A2B3C1D2.
Conclusion:
Compared with traditional extraction, ultrasonic method can save time, be easy to operate, improve extraction rates and need no heating.
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