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A two-dimensional YAC pooling strategy for library screening via STS and Alu-PCR methods
C T Amemiya1, M J Alegria-Hartman, C Aslanidis
1Lawrence Livermore National Laboratory, Biomedical Sciences Division, Livermore, CA 94551.
Nucleic Acids Research
|May 25, 1992
Abstract:
We have developed a simple two-dimensional YAC pooling strategy to facilitate YAC library screening via STS and Alu-PCR approaches. The method has been implemented using the human total genomic YAC library of Olson and coworkers, and its validity tested by isolation of many chromosomes 19- and 21-specific YACs. The Alu-PCR approach is notable in that it is hybridization-based, such that PCR primer pairs do not need to be repeatedly synthesized and tested for each screening step.