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Mixed alkanethiol self-assembled monolayers as substrates for microarraying applications.
Sammy S Datwani1, Ravi A Vijayendran, Emilie Johnson
1Caliper Life Sciences Incorporated, 605 Fairchild Drive, Mountain View, California 94043, USA.
Langmuir : the ACS Journal of Surfaces and Colloids
|June 30, 2005
Summary
Self-assembled monolayers (SAMs) improve microarray spot quality by controlling substrate surface properties. These stable, reproducible coatings enhance data quality in microarraying experiments.
Area of Science:
- Materials Science
- Biotechnology
- Surface Chemistry
Background:
- Microarray data quality heavily relies on the quality of printed spots.
- Contact printing techniques link spot quality to substrate surface characteristics.
Purpose of the Study:
- To investigate the use of self-assembled monolayers (SAMs) of mixed alkanethiols for controlling microarray substrate surface properties.
- To enhance microarray spot and data quality through surface modification.
Main Methods:
- Fabrication of surface coatings using mixed alkanethiol SAMs.
- Characterization of coatings via X-ray photoelectron spectroscopy and contact angle measurements.
- Printing and characterization of microarrays on SAM-coated substrates using metrology and fluorescence scanning.
Main Results:
- Mixed alkanethiol SAMs provide spatially homogeneous and reproducible surface characteristics.
- The surface coatings demonstrate stability and robustness over time.
- Utilizing SAMs as a surface coating significantly enhances microarray spot quality.
Conclusions:
- Mixed alkanethiol SAMs are effective surface coatings for microarray substrates.
- These SAMs improve spot quality, leading to enhanced data quality in microarraying.
- The developed surface modification technique offers a reproducible and stable solution for microarray applications.