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Traceability in fluorometry--Part I: Physical standards.

J Hollandt1, R D Taubert, J Seidel

  • 1Physikalisch-Technische Bundesanstalt, Abbestrasse 2-12, D-10587 Berlin, Germany. joerg.hollandt@ptb.de

Journal of Fluorescence
|June 30, 2005
PubMed
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Accurate fluorescence data comparability relies on correcting spectral measurements. This study details traceability chains from primary standards to spectroscopic techniques, ensuring reliable scientific data.

Area of Science:

  • Radiometry
  • Spectroscopy
  • Metrology

Background:

  • Ensuring consistent fluorescence data across instruments and labs is crucial.
  • Current methods require corrections for spectral irradiance and responsivity.
  • Traceability to primary standards is essential for reliable measurements.

Purpose of the Study:

  • To discuss the traceability chain in fluorometry from a radiometrist's perspective.
  • To present methods for correcting fluorescence spectra for accurate comparability.
  • To characterize transfer standards used in spectroscopic techniques.

Main Methods:

  • Realization of spectral radiance and responsivity scales using blackbody radiators, electron storage rings, and cryogenic radiometers.
  • Control of scales through key comparisons by national metrology institutes.

Related Experiment Videos

  • Characterization of source and detector transfer standards (tungsten strip lamps, integrating spheres, trap detectors).

    • Main Results:

    • Established traceability chains for fluorometry, linking primary standards to spectroscopic measurements.
    • Detailed characterization of transfer standards with state-of-the-art uncertainties.
    • Procedures for correcting wavelength- and polarization-dependent spectral properties.

    Conclusions:

    • A robust traceability framework is presented for accurate and comparable fluorescence measurements.
    • The methods ensure that spectroscopic data is traceable to fundamental radiometric quantities.
    • This work enhances the reliability of fluorescence spectroscopy in scientific research.