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Updated: Aug 17, 2026

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System
Published on: November 5, 2013
[Production and releasing sites of superoxide anion in phytopathogenic bacteria]
Xin Li1, Hong-yu Li, Xin-yue Pang
1School of Life Sciences, Lanzhou University, Lanzhou 730000, China. lixin2004@st.lzu.edu.cn
Abstract:
Many phytopathogenic bacteria were founded to release the superoxide anion (O2-) by themselves from different sites and the production of O2- was related to the pathogenicity of strains. The presences of O2- in cytomembrane, cell wall and filtrate were confirmed by chemical method and electron spin resonance (ESR). The results show that filtrate may be the primary site of O2- production due to its higher O2- generating activity either in the presence or the absence of DDC. Additional lines of evidence further suggested a regulatory mechanism of O2- production in phytopathogenic bacteria.
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