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Updated: Aug 17, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Accelerating bacterial identification by infrared spectroscopy by employing microarray deposition of microorganisms
Sufian F Al-Khaldi1, Magdi M Mossoba, Ashraf A Ismail
1Division of Microbiological Studies, OPDFB, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland 20740-3855, USA. Sufian.Al-Khaldi@cfsan.fda.gov
Abstract:
A microarray method for the deposition of bacteria onto an agar slide was developed to accelerate the formation of microcolonies. Representative microarrays each consisting of 40 micro-spots of five replicates of eight foodborne bacteria (Yersinia enterocolitica, Staphylococcus aureus, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Citrobacter freundii, Klebsiella pneumoniae, and Escherichia coli) were printed on a Brain Heart Infusion (BHI) agar slide using a contact micro-spotting robotic system. Within 3 h, sufficient bacterial cells were obtained to allow accurate identification of the microorganism by infrared spectroscopy. This approach allows a "complete-in-a-single-day" analysis of a large array of samples.
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