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Updated: Aug 11, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
The identification of a tandem H-DNA structure in the c-myc nuclease sensitive promoter element
A B Firulli1, D C Maibenco, A J Kinniburgh
1Department of Molecular Diagnostics, Roswell Park Cancer Institute, Buffalo, NY 14263.
Abstract:
Previous studies have shown that the c-myc nuclease sensitive element (NSE) is capable of forming H-DNA in vitro. The NSE sequence exhibits strong purine/pyrimidine strand asymmetry. To study the NSE further, we have isolated the element from other c-myc sequences and have shown that the NSE alone is sufficient for the formation of H-DNA in supercoiled plasmids. We also show that the NSE forms a complex structure containing both H-y3 and H-y5 H-DNA. We term this structure tandem H-DNA.
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