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[Analysis of Genotyping and Polymorphism of 104 RhD Variant Blood Donors in Tianjin]
Li-Na Wu1, Lei Ma1, Shuang-Yu Li1
1Immunology Laboratory of Tianjin Blood Center, Tianjin 300110, China.
Objective:
To investigate the genotyping and polymorphism of RhD variants in Tianjin region, providing reference for guiding clinical transfusion and partial pregnatal testing.
Methods:
Blood samples were collected from donors with serologically weak D phenotypes through laboratory testing from 2021 to 2024 at the Tianjin Blood Center. RHD genotyping was performed by using SSP-PCR. For unresolved genotypes, DNA sequence-based typing (SBT) was applied for gene sequencing.
Results:
A total of 104 RhD variants were statistically analyzed, and 72 were successfully indentified by RHD gene typing. Common variants included weak D type 15 (37 cases), DEL 1227A (16 cases), and DVI type (13 cases). Sequencing resolved 32 cases, predominantly weak D type 33. Two novel RHD genotypes not yet cataloged by ISBT were identified: one carried a homozygous 1100T>A mutation in exon 8, and the other showed a homozygous 526G>A mutation in exon 4 of the RHD gene.
Conclusion:
RHD exon structures exhibit polymorphism. The predominant RhD variants were weak D type 15, DEL 1227A, and weak D type 33 identified among blood donors in Tianjin.
