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Updated: Aug 17, 2026

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System
Published on: March 15, 2011
Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane
D Vigo1, S Villani, M Faustini
1Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Milan, Italy.
Abstract:
Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.
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