Comparison of the ratio HDL-cholesterol/total cholesterol on the Reflotron vs. conventional wet chemistry methods

A new HDL-cholesterol assay using solid phase reagent chemistry on the Reflotron has become available recently. This raises the question of whether the Reflotron now is suited for the determination of the ratio, HDL-cholesterol/total cholesterol, as a supplement to the lipid profile, viz. cholesterol, triacylglycerol and HDL-cholesterol. We investigated this ratio, which is an additional measure for the risk of coronary heart disease and found a good agreement between the Reflotron and conventional wet chemistry methods. We conclude that the Reflotron can be used for establishing the complete lipid profile of a patient.