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Updated: Aug 17, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
Fixation of conserved sequences shapes human intron size and influences transposon-insertion dynamics
Manuela Sironi1, Giorgia Menozzi, Giacomo P Comi
1Scientific Institute IRCCS E, Medea, Via Don Luigi Monza 20, 23842 Bosisio Parini (LC), Italy.
Abstract:
The basis for intron expansion in humans is largely unexplored. In this article, we demonstrate that intron expansion has primarily been determined by fixation of multispecies conserved sequences (MCSs) over time. The presence of MCSs has shaped intron features: the insertion of transposable elements (TEs) has been constrained as more MCSs were fixed. Analysis of TE and MCS distribution suggested an unprecedented estimate of information requirements for proper splicing of long introns with indication of sequence constraints extending up to >3 kb downstream 5' splice sites.
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