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Published on: January 20, 2016
Functionalized head-to-head hairpin polyamides: synthesis, double-stranded DNA-binding activity and affinity
Ludovic Halby1, Vladimir A Ryabinin, Alexandre N Sinyakov
1USM 0503-Régulation et Dynamique des Génomes, Muséum National d'Histoire Naturelle, Paris, France.
Abstract:
A series of 4 functionalized head-to-head-linked hairpin oligo(N-methylpyrrole) carboxamides with different linkers have been synthesized. Their ability to bind double-stranded DNA and sequence specificity were compared and the apparent Kd values of their DNA complexes were determined. These compounds, particularly those with iminodiacetic linkers, revealed a high affinity for DNA (Kd = 4.5-4.8 x 10(-9) M) and sequence specific recognition of 9-10 base pairs.
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