Related Experiment Video
Updated: Aug 17, 2026
14:04
Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
Published on: February 1, 2010
Identification of cellular membrane proteins interacting with hepatitis B surface antigen using yeast split-ubiquitin
Qi Chun Toh1, Tuan Lin Tan, Wei Qiang Teo
1Hepatitis Viruses and Liver Cancer Research Laboratory, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive 05N-10, Singapore 637551.
International Journal of Medical Sciences
|July 12, 2005
Summary
Researchers identified cellular proteins interacting with Hepatitis B surface antigen (HBsAg), a key component of Hepatitis B virus (HBV). These interactions are crucial for understanding HBV morphogenesis and viral replication.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Hepatitis B surface antigen (HBsAg) is a major envelope component of Hepatitis B virus (HBV).
- HBsAg, an endoplasmic reticulum resident membrane protein, is vital for viral morphogenesis.
- Limited knowledge exists regarding cellular proteins interacting with HBsAg and influencing HBV morphogenesis.
Purpose of the Study:
- To identify cellular membrane proteins that interact with Hepatitis B surface antigen (HBsAg).
- To elucidate the role of these interacting proteins in Hepatitis B virus (HBV) morphogenesis.
Main Methods:
- Utilized the yeast split-ubiquitin system for identifying protein-protein interactions.
- Screened for cellular membrane proteins interacting with HBsAg.
Main Results:
- Isolated several cellular membrane proteins interacting with HBsAg.
- Identified proteins including thioredoxin-related transmembrane protein 2, an adaptor protein involved in clathrin-mediated endocytosis and CD4 downregulation, and a coxsackie B virus co-receptor.
- These findings suggest potential roles in viral morphogenesis.
Conclusions:
- The study identified novel cellular membrane proteins interacting with HBsAg.
- These interactions offer new insights into the mechanisms of Hepatitis B virus (HBV) morphogenesis.
- Further functional analysis is warranted to fully understand the role of these proteins in HBV replication.
