Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identification, expression pattern, and subcellular location of human RIP isoforms.

Jin-Zhong Chen1, Sheng-Dong Huang, Chao-Neng Ji

  • 1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.

DNA and Cell Biology
|July 13, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial expression of concern: Role and fate of SP100 protein in response to Rep-dependent nonviral integration system.

Applied microbiology and biotechnology·2026
Same author

[The Enhancement Research of Magnetic Stirring with Laser Irradiation Aqueous Solution on ICP Source Radiation].

Guang pu xue yu guang pu fen xi = Guang pu·2018
Same author

Phyllanthus emblica Linn. fruit extract potentiates the anticancer efficacy of mitomycin C and cisplatin and reduces their genotoxicity to normal cells in vitro.

Journal of Zhejiang University. Science. B·2017
Same author

A simpler and more cost-effective peptide biosynthetic method using the truncated GST as carrier for epitope mapping.

PloS one·2017
Same author

Association between the MTHFR C677T polymorphism, blood folate and vitamin B12 deficiency, and elevated serum total homocysteine in healthy individuals in Yunnan Province, China.

Journal of the Chinese Medical Association : JCMA·2017
Same author

Epitomics: IgG-epitome decoding of E6, E7 and L1 proteins from oncogenic human papillomavirus type 58.

Scientific reports·2016

Researchers identified nine human RIP (hRIP) isoforms, revealing their diverse structures and nuclear localization. This study clarifies the genomic organization and expression patterns of hRIP, crucial for understanding nuclear import.

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • XRIP alpha, an adapter protein, is implicated in RAP nuclear import.
  • Mammalian homologs of hRIP exist, but their expression and genomic organization remain unclear.

Purpose of the Study:

  • To characterize the isoforms, genomic organization, and expression patterns of human RIP (hRIP).
  • To investigate the cellular localization of hRIP and its potential role in nuclear import.

Main Methods:

  • Isolation of nine hRIP isoforms from a human fetal brain library.
  • Bioinformatic analysis for genomic organization.
  • Tissue expression analysis across 16 human tissues.
  • EGFP-hRIP fusion protein localization in HEK293 cells.

Related Experiment Videos

Main Results:

  • Nine hRIP isoforms were identified, varying in structural domains (Zn finger-like structures, acidic regions).
  • hRIP gene comprises seven exons and is located on human chromosome 17p13.
  • hRIP is ubiquitously expressed across 16 human tissues.
  • All EGFP-hRIP fusion proteins localized to the nucleus in HEK293 cells.

Conclusions:

  • The diverse hRIP isoforms possess distinct structural features, potentially influencing their function.
  • hRIP's genomic organization and widespread expression suggest a fundamental role in cellular processes.
  • The nuclear localization of hRIP supports its involvement in nuclear import mechanisms.