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Updated: Jul 26, 2026
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
[Experimental study of an animal model of diffuse panbronchiolitis]
1Department of Pulmonary Medicine, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China.
Objective:
To establish an animal model of diffuse panbronchiolitis (DPB) and to investigate the pathogenesis of DPB.
Methods:
Sprague-Dawley (SD) rats were intubated with a silicone tube pre-coated with Pseudomonas aeruginosa (P. aeruginosa) in the bronchus. After sixty days, the pathological changes of lung tissue were analyzed.
Results:
Pathological changes characteristic of DPB were found in the SD rats after the placement of a silicate tube pre-coated with P. aeruginosa in the bronchus. The lung pathology was characterized by diffuse inflammation around bronchi and bronchioles in both lungs, and thickening of the walls of bronchioles with infiltration of lymphocytes, plasma cells, and histiocytes. In addition, bronchus-associated lymphoid tissue (BALT) hyperplasia and accumulation of foamy cells were observed around the bronchi. Thickened walls and enlarged lumen of pulmonary arteries were also noticed occasionally.
Conclusion:
The SD rat model of DPB could be established by infection of P. aeruginosa.
