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Polyglycolic acid/chitosan glue and apoptosis of endometriotic cells
Yu-Chi Wang1, Ru-Huei Fu, Hsyue-Jen Hsieh
1Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
Objective:
To induce apoptosis of endometriotic cells of patients with endometriosis.
Design:
To demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis.
Setting:
University hospital infertility center.
Patient(S):
Twelve women who visited the center for infertility therapy.
Intervention(S):
Polyglycolic acid/chitosan glue was applied into primary endometriotic cells; the manipulated cells were collected 1-4 days after polyglycolic acid/chitosan glue treatment.
Main Outcome Measure(S):
Primary endometriotic cell cultures from eutopic endometriotic tissue were established. The effect of the novel biological glue, polyglycolic acid/chitosan glue A, on endometrial cells in vitro was examined. The different stages of apoptosis were analyzed using flow cytometry with fluorescein isothiocyanate conjugate (FITC)-annexin V and propidium iodide staining.
Result(S):
The growth inhibitory effects of polyglycolic acid/chitosan glue A on endometrial cells were found to be dose-response and time dependent. Less than 15% viability was detected in cultures containing 2,000 microg of polyglycolic acid/chitosan glue A after 4 days of treatment. Induced apoptosis and caspase activity were revealed. The caspase-3 activity increased 2.2-fold with 4 days of culture with 2,000 microg of polyglycolic acid/chitosan glue A.
Conclusion(S):
This is the first study to demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis, thus suggesting that this new biological glue may be useful for endometriosis therapy.
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