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Cytotoxicity of substances leached or dissolved from pulp capping materials
B N Cavalcanti1, S M Rode, M M Marques
1Department of Endodontics, School of Dentistry, University of São Paulo, São Paulo, Brazil. bncavalcanti@uol.com.br
International Endodontic Journal
|July 14, 2005
Summary
Substances from an adhesive system showed cytotoxicity to human dental pulp fibroblasts, while calcium hydroxide proved biocompatible in this cell culture study.
Area of Science:
- Dental Materials Science
- Biocompatibility Testing
- Cell Biology
Background:
- Pulp capping materials are crucial for preserving dental pulp vitality.
- Assessing the biocompatibility of these materials is essential for successful endodontic treatments.
- Understanding the cytotoxic effects of leached or dissolved substances is vital.
Purpose of the Study:
- To evaluate the cytotoxic effects of substances from pulp capping materials on human pulp fibroblasts.
- To compare the biocompatibility of calcium hydroxide, an adhesive resin, and orthophosphoric acid.
Main Methods:
- Human pulp fibroblasts were exposed to conditioned media from tested materials.
- Cytotoxicity was assessed using Trypan blue dye exclusion assay for cell viability and survival.
- Statistical analysis included ANOVA and Tukey's test to determine significance.
Main Results:
- Calcium hydroxide showed no significant impact on cell viability or growth.
- The adhesive resin significantly reduced cell viability, dropping to 10% by 12 hours.
- Orthophosphoric acid showed transient cytotoxicity only at the initial time point (0 h).
Conclusions:
- Substances dissolved from the tested adhesive system demonstrated cytotoxicity towards human dental pulp fibroblasts.
- Leached substances from calcium hydroxide exhibited biocompatible properties in this in vitro study.