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Updated: Aug 17, 2026
Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
Published on: April 6, 2019
Trypanosoma vivax: a novel method for purification from experimentally infected sheep blood
L E González1, J A García, C Núñez
1Universidad Simón Bolívar, Departamento de Biología Celular, Grupo deBioquímica e Inmunología de Hemoparásitos, Caracas, Venezuela.
Abstract:
Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts.
