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Related Experiment Videos

Thin-film IrOx pH microelectrode for microfluidic-based microsystems.

Igor A Ges1, Borislav L Ivanov, David K Schaffer

  • 1Department of Biomedical Engineering, Vanderbilt University, 6301 Stevenson Center, VU Station B 351631, Nashville, TN 37235-1631, USA.

Biosensors & Bioelectronics
|July 19, 2005
PubMed
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Researchers developed tiny iridium oxide pH microsensors for monitoring cell metabolism in nanoliter volumes. These novel microsensors enable real-time measurement of cell acidification rates in microfluidic cell culture systems.

Area of Science:

  • Biosensors and Microfluidics
  • Materials Science and Engineering
  • Cellular Metabolism Monitoring

Background:

  • Microsensors are essential for real-time monitoring of cellular metabolism in small volumes.
  • Existing technologies face limitations in sensitivity and integration for nanoliter-scale cell cultures.

Purpose of the Study:

  • To fabricate and characterize novel thin-film iridium oxide (IrOx) pH microsensors for on-chip cell culture.
  • To demonstrate the application of these microsensors in measuring cellular acidification rates in nanoliter volumes.

Main Methods:

  • Fabrication of planar IrOx thin-film pH microsensors (20x20 µm and 20x40 µm) on platinum microelectrodes via electrochemical deposition.
  • Characterization of sensor response, including slope, pH range, response time, and long-term stability.

Related Experiment Videos

  • Integration of microsensors into a microfluidic chip for on-chip cell culture (25 nL) and measurement of Chinese Hamster Ovary (CHO) and fibroblast cell acidification.

    • Main Results:

    • Anodic IrOx films exhibited a near super-Nernstian pH response (-77.6±2 mV/pH) within the 4-11 pH range at 22°C.
    • Microsensors demonstrated rapid response times as low as 6 seconds.
    • Minimal baseline drift (2-3 mV/month) was observed, manageable through calibration.
    • Successful real-time measurement of cell acidification rates in 25 nL volumes was achieved.

    Conclusions:

    • Developed highly sensitive and rapid-response IrOx pH microsensors suitable for nanoliter-scale cell culture.
    • Demonstrated the first use of planar IrOx microelectrodes for on-chip monitoring of cellular acidification in microfluidic systems.
    • These microsensors offer a promising tool for advanced cell metabolism studies and drug screening applications.