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Bacteria detection using disposable optical leaky waveguide sensors.

Mohammed Zourob1, Stephan Mohr, Bernard J Treves Brown

  • 1School of Chemical Engineering and Analytical Science, The University of Manchester, PO Box 88, Sackville street, Manchester, M60 1QD, UK.

Biosensors & Bioelectronics
|July 19, 2005
PubMed
Summary
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New disposable leaky waveguide sensor devices (LWD) offer sensitive detection of pathogenic bacteria. These cost-effective sensors enhance light interaction with captured bacteria, outperforming surface plasmon resonance (SPR) in illumination intensity.

Area of Science:

  • Biophotonics
  • Biosensing
  • Nanotechnology

Background:

  • Pathogenic bacteria detection is crucial for public health.
  • Existing biosensing methods can be expensive and complex.
  • Leaky waveguide sensors offer potential for sensitive, low-cost detection.

Purpose of the Study:

  • To develop novel disposable absorbing material clad leaky waveguide sensor devices (LWD) for pathogenic bacteria detection.
  • To enhance the interaction of light with captured bacteria for increased sensitivity.
  • To evaluate the performance of LWDs compared to existing technologies like SPR.

Main Methods:

  • Fabrication of disposable LWD chips at room temperature without expensive equipment.
  • Immobilization of antibodies on the sensor surface to capture bacteria.

Related Experiment Videos

  • Characterization of LWDs by detecting refractive index changes, scattering, and fluorescence from bacterial spores.
  • Illumination of captured bacteria at the coupling angle.

    • Main Results:

    • LWDs demonstrated effective detection of Bacillus subtilis var. niger (BG) bacterial spores.
    • The detection limit for BG spores was 10(4) spores/ml.
    • Illumination intensity generated by LWDs was three times greater than that of SPR.

    Conclusions:

    • Disposable LWDs are a promising, cost-effective technology for sensitive pathogenic bacteria detection.
    • The enhanced evanescent field interaction in LWDs leads to superior performance.
    • LWDs offer a viable alternative to traditional biosensing methods for various applications.