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Updated: Aug 16, 2026
A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer
Published on: March 6, 2018
Annotation of androgen dependence to human prostate cancer-associated genes by microarray analysis of mouse prostate
Jas Singh1, Pachiappan Manickam, Michael Shmoish
1Department of Medicine, D06, The University of Sydney, NSW 2006, Australia.
Abstract:
In silico methods and array technologies have identified genes differentially expressed in prostate cancer. Biological functions of the identified genes are often unclear. Considering the biological significance of androgens in prostate cancer, we profiled the prostate transcripts of congenital androgen-deficient mice with or without androgen replacement in vivo using murine gene expression array. In parallel genes differentially expressed in human prostate cancer were identified by Digital Differential Display and the Serial Analysis of Gene Expression. Androgen dependence of the identified genes was then determined by the steady-state mRNA levels of the murine orthologs in response to androgen treatment. The annotation is supported by the finding that some of the androgen target genes have been reported previously with independent experiments.
Insights
This study identifies androgen-regulated genes in prostate cancer using mouse models and human data. Understanding these genes helps clarify the biological functions of androgens in prostate cancer development.
Area of Science:
- Molecular Biology
- Genomics
- Oncology
Background:
- Prostate cancer is influenced by androgens, but the specific genes regulated by androgens remain unclear.
- Existing methods for identifying differentially expressed genes in prostate cancer often do not elucidate their biological functions.
Purpose of the Study:
- To identify androgen-dependent genes in prostate cancer.
- To clarify the biological functions of genes implicated in prostate cancer development.
Main Methods:
- Gene expression profiling of prostate tissues from androgen-deficient mice with and without androgen replacement.
- Analysis of human prostate cancer gene expression data using Digital Differential Display and Serial Analysis of Gene Expression.
- Determination of androgen dependence by measuring mRNA levels of murine orthologs.
Main Results:
- Identified genes differentially expressed in prostate cancer.
- Determined the androgen dependence of these genes through mRNA level analysis in mice.
- Validated findings by confirming previously reported androgen target genes.
Conclusions:
- This study successfully identified androgen-regulated genes relevant to prostate cancer.
- The findings contribute to understanding the role of androgens in prostate cancer biology and provide potential targets for further research.
