Teaching third-year students MALDI-TOF using a combination of online interactive simulation and face-to-face...

Area of Science:

• Clinical Microbiology
• Medical Education
• Diagnostic Technology

Background:

• Accurate identification of disease-causing microorganisms is crucial in clinical microbiology.
• Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF) is a key technology for microbial identification.
• Limited access to MALDI-TOF equipment hinders hands-on training for students in many educational settings.

Purpose of the Study:

• To evaluate the effectiveness of a blended learning approach using an online MALDI-TOF simulation for teaching diagnostic microbiology.
• To provide students with practical experience in microbial identification techniques despite equipment limitations.

Main Methods:

• Implemented a blended teaching strategy combining in-person practical sessions with an interactive online MALDI-TOF mass spectrometry simulation.
• Students processed virtual patient samples, performed microbiological tests, and used the simulation to generate and interpret MALDI-TOF data.
• Assessed learning outcomes through written laboratory reports and student questionnaires.

Main Results:

• Students showed significant improvements in self-reported understanding of MALDI-TOF principles and application.
• Nearly all participants successfully passed the written assessment, indicating effective knowledge acquisition.
• The simulation was perceived as enjoyable, interactive, and conducive to authentic learning.

Conclusions:

• Online simulations offer a cost-effective and scalable solution for training students in advanced diagnostic techniques like MALDI-TOF.
• This approach effectively bridges the gap in practical education caused by limited access to expensive laboratory equipment.
• Simulations enhance student engagement and provide valuable hands-on experience in diagnostic microbiology.