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Calcium secretion into milk.

Margaret C Neville1

  • 1University of Colorado Health Sciences Center at Fitzsimmons, Aurora, 80010, USA.

Journal of Mammary Gland Biology and Neoplasia
|July 19, 2005
PubMed
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Milk calcium is complexed with citrate, phosphate, and casein. Calcium transport into milk involves the Golgi and calcium ATPases, but basolateral transport mechanisms remain unknown.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Dairy Science

Background:

  • Milk calcium exists around 3 mM, forming complexes with citrate, phosphate, and casein.
  • Milk calcium levels are regulated by citrate and casein, not free calcium ion (Ca2+) concentrations.
  • Calcium in milk is primarily transported via exocytosis of Golgi-derived secretory vesicles.

Purpose of the Study:

  • To investigate the mechanisms of calcium transport in milk production.
  • To identify the specific calcium transporters involved in milk secretion.
  • To explore the regulation of calcium homeostasis in mammary alveolar cells.

Main Methods:

  • Analysis of calcium complex formation in milk.
  • Investigation of gene expression for calcium ATPases (PMCA2bw, SPCA) during lactation.

Related Experiment Videos

  • Review of existing literature on calcium transport in mammary cells.

    • Main Results:

    • Calcium forms significant complexes with citrate, phosphate, and casein in milk.
    • Gene expression for PMCA2bw and SPCA is upregulated during lactation, suggesting their involvement in calcium transport.
    • Mechanisms of calcium transport across the basolateral membrane of alveolar cells are currently unknown.

    Conclusions:

    • Calcium complexation is key to its high concentration in milk.
    • Specific calcium ATPases are likely involved in milk calcium secretion.
    • Further research is needed to elucidate basolateral calcium transport in mammary alveolar cells.