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Development of cell culture (MDCK) live cold-adapted (CA) attenuated influenza vaccine

Y Z Ghendon1, S G Markushin, I I Akopova

  • 1Research Institute for Virus Preparation, 1 Dubrovskaya str 15, 115088 Moscow, Russia. misha_vasiliev@hotmail.com

Vaccine
|July 20, 2005
PubMed

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Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...