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Updated: Aug 16, 2026
Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
Published on: June 3, 2012
[Clone expression and purification of influenza A virus nucleoprotein gene]
Shi-song Fang1, Xiao-wen Cheng, Tao Liu
1Department of Food and Bioengineering, South China University of Technology, Guangzhou 510642, China.
Objective:
To clone the influenza A virus NP gene into expression vector and to purify the target protein, which was used to study the preparation of monoclonal antibody.
Methods:
The RNA of influenza A virus was extracted and primers were designed according the NP gene sequence, then the NP gene of influenza A virus was expressed in E.coli DH5alpha and the NP protein was purified by affinity chromatography.
Results:
The recombinant expression vector-pGEX-4T-2-NP was successfully constructed and relatively pure target protein was obtained.
Conclusion:
Through reasonably controlling the fermentation time, growth temperature and induction concentration, satisfactory soluble target product was obtained.
