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Assessment of RET/PTC oncogene activation in thyroid nodules utilizing laser microdissection followed by nested

Giovanni Tallini1, Guilherme Brandao

  • 1Division of Pathology, Yale University School of Medicine, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 21, 2005
PubMed

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...