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Resveratrol regulates insulin-like growth factor-II in breast cancer cells

Sharda Vyas1, Yayesh Asmerom, Daisy D De León

  • 1Department of Anatomy, CSP 11012, Loma Linda University, Loma Linda, California 92350, USA.

Endocrinology
|July 23, 2005
PubMed

Insights

Resveratrol (RSV) affects Insulin-like Growth Factor-II (IGF-II) in breast cancer cells. This study shows IGF-II mediates RSV

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cancer Research

Background:

  • Insulin-like Growth Factor-II (IGF-II) is a key regulator in breast cancer, influencing cell proliferation and apoptosis.
  • Resveratrol (RSV), a phytoestrogen, exhibits anti-cancer properties and interacts with estrogen pathways.
  • Estrogen's role in regulating IGF-II suggests a potential link between RSV and IGF-II in breast cancer.

Purpose of the Study:

  • To investigate if RSV modulates IGF-II expression and secretion in breast cancer cells.
  • To determine if RSV's effect on IGF-II is dependent on estrogen receptor (ER) status.
  • To ascertain whether IGF-II mediates the effects of RSV on breast cancer cell growth and survival.

Main Methods:

  • Treatment of ER-positive (MCF-7, T47D) and ER-negative (MCF-10) breast cancer cells with varying concentrations of RSV.
  • Analysis of precursor IGF-II mRNA and protein levels.
  • Assessment of cell growth inhibition and survival using IGF-I receptor blocking antibodies and tamoxifen.
  • Evaluation of IGF-I levels.

Main Results:

  • Low-dose RSV (10^-6 M) stimulated IGF-II in ER-positive cells, an effect blocked by 17beta-estradiol.
  • RSV-stimulated growth was inhibited by an IGF-I receptor antibody or tamoxifen.
  • High-dose RSV (10^-4 M) inhibited IGF-II secretion and cell growth in ER-positive cells.
  • RSV inhibited cell growth in ER-negative cells, but IGF-II levels did not increase.

Conclusions:

  • Resveratrol differentially regulates IGF-II in breast cancer cells, with effects dependent on concentration and ER status.
  • IGF-II plays a mediating role in both the growth-promoting and growth-inhibitory effects of RSV on breast cancer cells.
  • These findings highlight a complex interaction between RSV, IGF-II, and ER signaling in breast cancer therapy and chemoprevention.

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