[Experimental studies on preparation and property of scaffold material of bio-derived bone loading wo-1]

To provide the chosen scaffold materials for experiment and application of tissue engineering and to detect the properties of the collagen bio-derived bone scaffold material loading WO-1.

The pure bio-derived bone scaffold material, bio-derived bone scaffold material loading collagen, collagen bio-derived bone scaffold material loading WO-1 were made by use of allograft bone, and type-I collagen, and WO-1. The morphological features, constitute components and mechanical properties were examined by scanning electron microscopy, X- rays diffraction and mechanical assay.

The bio-derived bone scaffold material maintained natural network pore system; the bio-derived bone scaffold material loading collagen maintained natural network pore system, the surface of network pore system was coated by collagen membrane; the collagen bio-derived bone scaffold material loading WO-1 maintained natural network pore system, the surface of network pore system was coated by collagen membrane. The pore sizes of the 3 materials were 90-700 microm, 75-600 microm and 80-600 microm, respectively, and the porosities were 87.96%, 80.47%, 84.2%. There was no significant difference between them (P>0.05). The collagen bio-derived bone scaffold material loading WO-1 consisted of [HA,Ca10 (OH)2 (PO4)6. There was no significant difference in the mechanical strength of the three scaffold materials.

The bio-derived bone scaffold material loading WO-1 is as good as bio-derived bone scaffold material and collagen bio-derived bone scaffold material, and it is an effective scaffold material for tissue engineering bone.