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Protective effect of membrane cofactor protein against complement-dependent injury
Dong Xu1, Shou-jian Huang, Jin-qun Wang
1Department of Pharmacology, Sun Yat-Sen Medical College of Sun Yat-Sen University, Guangzhou 510080, China.
Acta Pharmacologica Sinica
|July 26, 2005
Summary
Membrane cofactor protein (MCP, CD46) demonstrates a protective role against complement-dependent injury. Studies show MCP inhibits platelet damage and prevents tissue injury in various experimental models.
Area of Science:
- Immunology
- Complement System Biology
Background:
- Complement-dependent injury is implicated in various pathological conditions.
- Membrane cofactor protein (MCP, CD46) is a complement regulatory protein.
Purpose of the Study:
- To evaluate the protective role of membrane cofactor protein (MCP, CD46) against complement-mediated damage.
Main Methods:
- MCP was isolated using ion exchange chromatography.
- Protective effects were assessed using models of cobra venom factor (CVF)-induced platelet activation, human serum-induced cardiac injury, and reverse passive Arthus reaction.
Main Results:
- MCP inhibited CVF-induced platelet metamorphosis with an IC50 of 56.7 mg/L.
- MCP prevented complement-induced injury in isolated working guinea pig hearts.
- MCP administration reduced skin lesions in a rat model of reverse Arthus reaction.
Conclusions:
- Membrane cofactor protein (MCP, CD46) exhibits a significant protective effect against complement-dependent injury.
- MCP demonstrates therapeutic potential in conditions involving complement activation.