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Protective effect of membrane cofactor protein against complement-dependent injury

Dong Xu1, Shou-jian Huang, Jin-qun Wang

  • 1Department of Pharmacology, Sun Yat-Sen Medical College of Sun Yat-Sen University, Guangzhou 510080, China.

Acta Pharmacologica Sinica
|July 26, 2005
PubMed
Abstract

Insights

Membrane cofactor protein (MCP, CD46) demonstrates a protective role against complement-dependent injury. Studies show MCP inhibits platelet damage and prevents tissue injury in various experimental models.

Area of Science:

  • Immunology
  • Complement System Biology

Background:

  • Complement-dependent injury is implicated in various pathological conditions.
  • Membrane cofactor protein (MCP, CD46) is a complement regulatory protein.

Purpose of the Study:

  • To evaluate the protective role of membrane cofactor protein (MCP, CD46) against complement-mediated damage.

Main Methods:

  • MCP was isolated using ion exchange chromatography.
  • Protective effects were assessed using models of cobra venom factor (CVF)-induced platelet activation, human serum-induced cardiac injury, and reverse passive Arthus reaction.

Main Results:

  • MCP inhibited CVF-induced platelet metamorphosis with an IC50 of 56.7 mg/L.
  • MCP prevented complement-induced injury in isolated working guinea pig hearts.
  • MCP administration reduced skin lesions in a rat model of reverse Arthus reaction.

Conclusions:

  • Membrane cofactor protein (MCP, CD46) exhibits a significant protective effect against complement-dependent injury.
  • MCP demonstrates therapeutic potential in conditions involving complement activation.

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