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Related Experiment Videos

Probing cytoskeleton modulation by optical biosensors.

Ye Fang1, Ann M Ferrie, Guangshan Li

  • 1Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14870, USA. fangy2@corning.com

FEBS Letters
|July 26, 2005
PubMed
Summary
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Resonant waveguide grating biosensors effectively measured cell mass changes, revealing insights into cytoskeleton structure. Actin disruption agents amplified mass loss, highlighting their impact on cellular integrity.

Area of Science:

  • Cell Biology
  • Biophysics
  • Biochemistry

Background:

  • The cytoskeleton plays a crucial role in maintaining cell structure and function.
  • Studying cytoskeleton dynamics requires sensitive and quantitative measurement techniques.
  • Resonant waveguide grating (RWG) biosensors offer label-free, real-time mass sensing capabilities.

Purpose of the Study:

  • To investigate the utility of RWG biosensors for analyzing cytoskeleton structure in cells.
  • To quantify mass changes in cells upon saponin treatment and in the presence of cytoskeleton modulators.
  • To assess the impact of actin disruption agents on cell mass dynamics.

Main Methods:

  • Chinese hamster ovary (CHO) cells were cultured and analyzed using RWG biosensors.
  • Cells were exposed to varying concentrations of saponin to induce plasma membrane permeabilization.

Related Experiment Videos

  • The effect of pre-treatment with actin disruption agents (cytochalasin B, latrunculin A) on saponin-induced mass changes was evaluated.

    • Main Results:

    • Saponin induced dose-dependent and dynamic mass changes in CHO cells.
    • Higher saponin concentrations (> 60 microg/ml) resulted in a net mass loss, attributed to the diffusion of intracellular components.
    • Pre-treatment with cytochalasin B or latrunculin A significantly enhanced the saponin-induced cell mass loss.
    • RWG biosensor measurements provided quantitative data on cytoskeleton-mediated mass retention.

    Conclusions:

    • RWG biosensors are a valuable tool for studying cell mass changes and cytoskeleton structure.
    • The biosensor platform can be used to screen for compounds that modulate cytoskeleton organization and function.
    • This approach offers a sensitive and label-free method for cell-based assays relevant to cytoskeleton research.