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Related Experiment Videos

A high-throughput 3-D composite dielectrophoretic separator.

Henry O Fatoyinbo1, David Kamchis, Reginald Whattingham

  • 1School of Engineering, University of Surrey, Surrey GU2 7XH, UK.

IEEE Transactions on Bio-Medical Engineering
|July 27, 2005
PubMed
Summary

This study introduces a novel dielectrophoresis device with improved electrode design for higher throughput. The new method effectively sorts viable and nonviable yeast cells, advancing bioprocessing applications.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Separation

Background:

  • Dielectrophoresis (DEP) shows promise in bioprocessing and medicine.
  • Current DEP devices face limitations due to low throughput and electrode scale.
  • High throughput is crucial for practical DEP applications.

Purpose of the Study:

  • To develop a novel dielectrophoresis (DEP) device with enhanced electrode construction.
  • To overcome the throughput limitations of conventional DEP systems.
  • To demonstrate efficient cell separation using the new DEP device.

Main Methods:

  • A novel drilled laminated structure was employed for electrode construction.
  • Electrodes were fabricated with dimensions of 30 micrometers across and 150 micrometers apart.

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  • The design features electrodes along all sides of the drilled bore to increase trapping efficiency.

    • Main Results:

    • The novel DEP device demonstrated improved trapping efficiency compared to conventional designs.
    • A separator was developed and tested using a 50:50 mixture of viable and nonviable yeast cells.
    • The device successfully sorted the yeast cell mixture into an 86:14 ratio at a throughput of 25 mL/hr.

    Conclusions:

    • The novel drilled laminated electrode structure significantly enhances dielectrophoresis performance.
    • This approach offers a viable solution for high-throughput cell separation.
    • The developed DEP separator has practical implications for bioprocessing and clinical diagnostics.