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Exhaled 8-isoprostane in childhood asthma
Sukhbir K Shahid1, Sergei A Kharitonov, Nicola M Wilson
1National Heart and Lung Institute, Imperial College London, London, UK. a.bush@ic.ac.uk
Respiratory Research
|July 27, 2005
Summary
Oxidative stress is elevated in children with asthma, as measured by exhaled 8-isoprostane. This biomarker indicates increased oxidative stress, even with inhaled steroid treatment, suggesting potential for antioxidant therapies.
Area of Science:
- Pediatric Pulmonology
- Biomarkers
- Oxidative Stress
Background:
- Exhaled breath condensate (EBC) offers a non-invasive method for assessing airway inflammation and oxidative stress.
- EBC may be valuable in evaluating childhood asthma.
Purpose of the Study:
- To measure exhaled 8-isoprostane, a marker of oxidative stress, in children with asthma.
- To compare oxidative stress levels in steroid-naïve asthmatic children, inhaled steroid-treated asthmatic children, and healthy controls.
Main Methods:
- Measured exhaled 8-isoprostane concentrations in EBC from children aged 5-17 years.
- Included steroid-naïve asthmatics (n=13), inhaled steroid-treated asthmatics (n=12), and healthy controls (n=11).
- Also measured exhaled nitric oxide (eNO) in the same groups.
Main Results:
- Significantly elevated exhaled 8-isoprostane in steroid-naïve asthmatics (9.3 pg/ml) and steroid-treated asthmatics (6.7 pg/ml) compared to healthy controls (3.8 pg/ml).
- Steroid-naïve asthmatics showed higher exhaled nitric oxide (eNO) (28.5 ppb) than controls (12.6 ppb), while treated asthmatics had similar eNO levels to controls.
- No correlation found between exhaled 8-isoprostane and asthma duration, inhaled steroid dose, or eNO levels.
Conclusions:
- Exhaled 8-isoprostane is elevated in asthmatic children, reflecting increased oxidative stress.
- Inhaled steroid therapy does not appear to normalize these elevated 8-isoprostane levels.
- 8-isoprostane is a promising non-invasive biomarker for oxidative stress in pediatric asthma, suggesting potential for future antioxidant therapies.