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Published on: September 15, 2016
Immunoassay signal amplification on glass slides based on electroless deposition
1School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China. suyanlei@tju.edu.cn
Abstract:
Saturated coverage of antibodies was first constructed on glass slides coated with polyelectrolyte multilayer; then sandwich immunoreactions were carried out. Following immunoreactions, the glass slides were immersed in a freshly prepared solution containing 0.01 wt% HAuCl4 and 0.4 mM NH2OH.HCl. The growth of immunogold nanoparticles on glass slides was monitored with UV-vis spectrometer in real time. The data indicated that the immunoassay signal amplification really depends on the time of electroless deposition process and the concentration of antigen.
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