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Related Experiment Videos

Methods and milliliter scale devices for high-throughput bioprocess design.

Dirk Weuster-Botz1, Robert Puskeiler, Andreas Kusterer

  • 1Lehrstuhl für Bioverfahrenstechnik, Technische Universität München, Boltzmannstr. 15, 85748, Garching, Germany. d.weuster-botz@lrz.tum.de

Bioprocess and Biosystems Engineering
|July 29, 2005
PubMed
Summary

Optimized gas-inducing bioreactors achieve high cell densities (16.5 g L(-1)) in parallel Escherichia coli cultivations. This system offers high oxygen transfer and reproducibility for automated, high-throughput bioprocessing.

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Process Optimization

Background:

  • Stirred-tank reactors are crucial for microbial cultivations.
  • High-throughput screening and optimization require efficient bioreactor systems.
  • Scalable and reproducible bioprocessing is essential for industrial applications.

Purpose of the Study:

  • To optimize gas-inducing impellers and magnetic drives for small-scale bioreactors.
  • To develop an automated system for parallel cultivation and monitoring.
  • To achieve high cell densities and reproducibility in high-throughput microbial cultivations.

Main Methods:

  • Electromagnetic and computational fluid dynamics simulations for impeller optimization.
  • Development of a 48-bioreactor system with magnetic inductive drives.

Related Experiment Videos

  • Online dissolved oxygen monitoring using a fluorescence reader and phase detection.
  • Self-optimizing scheduling software for automated liquid handling, titration, and sampling.
  • Parallel batch cultivations of Escherichia coli with varying media compositions.

    • Main Results:

    • Achieved high impeller speeds (up to 4,000 rpm) with low power input (63 W for 48 bioreactors).
    • Local energy dissipation comparable to standard stirred-tank bioreactors (up to 50 W L(-1)).
    • High cell densities (up to 16.5 g L(-1) dry cell mass) achieved within 5 hours.
    • Demonstrated high parallel reproducibility (standard deviation < 3.5%) without pH control.
    • High oxygen transfer capability confirmed for the gas-inducing stirred-tank bioreactors.

    Conclusions:

    • The optimized gas-inducing stirred-tank bioreactors enable efficient, high-throughput microbial cultivations.
    • The automated system provides high reproducibility and oxygen transfer for achieving high cell densities.
    • This technology is suitable for rapid screening of media compositions and optimizing bioprocesses.