Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

p63 regulates commitment to the prostate cell lineage.

Sabina Signoretti1, Maira M Pires, Meghan Lindauer

  • 1Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. ssignoretti@partners.org

Proceedings of the National Academy of Sciences of the United States of America
|July 30, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Progenitor Resilience and the Early Onset of Chronic Lung Diseases: NHLBI workshop report.

American journal of respiratory cell and molecular biology·2026
Same author

Advancing Esophageal Disease Modeling: Microfluidic Platforms for Adult Tissue-Resident Stem Cell Culture and Differentiation.

Gastro hep advances·2026
Same author

Reply.

Gastroenterology·2025
Same author

A Reflux Linked GATA Factor Fulcrum Dictates Lineage Commitment Through GPRC5B During the Esophageal Dysplastic Transition.

Cellular and molecular gastroenterology and hepatology·2025
Same author

<i>RAD21</i> promotes oncogenesis and lethal progression of prostate cancer.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

The polyunsaturated fatty acid docosahexaenoic affects mitochondrial function in prostate cancer cells.

Cancer & metabolism·2024
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Prostate secretory cells arise from p63-positive basal progenitors. However, urothelial umbrella cells can develop independently of p63, revealing distinct lineage requirements in urinary tract development.

Area of Science:

  • Developmental Biology
  • Urogenital System Development
  • Cell Lineage Specification

Background:

  • The molecular mechanisms governing prostate and urothelial development are poorly understood.
  • This knowledge gap hinders the identification of cell types and molecular events in prostate and bladder cancer.
  • Mice lacking the basal cell marker p63 exhibit epithelial defects, including prostate agenesis and urothelial abnormalities.

Purpose of the Study:

  • To utilize the p63-/- mouse model to define cell lineages within the prostate epithelium and urothelium.
  • To investigate the role of p63 in maintaining prostate cell lineage commitment.
  • To elucidate the developmental independence of urothelial umbrella cells.

Main Methods:

  • Generation of p63-/- mouse chimeras by complementing p63-/- blastocysts with p63+/+ beta-galactosidase (beta-gal)-positive ES cells.

Related Experiment Videos

  • Urogenital sinus transplantation experiments.
  • Analysis of cell lineage contribution and differentiation in chimeric and transplanted tissues.

    • Main Results:

    • Secretory cells of the prostate originate from p63-positive basal progenitor cells.
    • p63 is essential for preventing intestinal differentiation of the urogenital sinus endoderm, maintaining prostate lineage commitment.
    • In the urothelium, umbrella cells can develop and be maintained independently of p63-positive basal and intermediate cells.

    Conclusions:

    • p63 plays a critical role in prostate epithelial development, specifically in basal progenitor cell differentiation into secretory cells.
    • p63 acts as a crucial determinant for prostate lineage, preventing alternative differentiation pathways.
    • Urothelial development, particularly umbrella cell formation, is less dependent on p63 compared to prostate development.